A. VSV-G-tagged MvaT (MvaT-V) and MvaU (MvaU-V) associate with intergenic and coding sequences of rsmZ (left panel) but not of rsmY (right panel). Genes corresponding to the enriched regions are represented below the charts above the horizontal line if their orientation is left to right, and below the horizontal line if their orientation is right to left.
B. Effect of mvaT and mvaU deletions on the expression of rsmZ (left panel) and rsmY (right panel), as measured by Miller assays using rsmZ-lacZ and rsmY-lacZ reporter fusions.
C. The region between 3' of the UAS site and transcription start site (+1) of rsmZ is significantly enriched for A and T compared to the overall composition of various P. aeruginosa genomes and compared to the rsmZ coding sequence.