Figure 2.

Bestrophin1 transcript inhibition decreases Ca²⁺-activated Cl⁻ current amplitude and expression in axotomized sensory neurons. A, Representative traces of voltage-gated Ca²⁺ current and CaCC recorded in axotomized neurons 3 d after 1 μm siCONTROL or siRNA Best1 electroporation. A ramp protocol from −80 to +40 mV activated the voltage-gated Ca²⁺ current, followed by an inward tail current at −80 mV, previously identified as CaCC. B, After candidate gene screening with siRNA interference, CaCC amplitude decreased exclusively in siRNA Best1-treated neurons. C, Analysis of the percentage of neurons expressing CaCC after gene screening with siRNA interference. *p < 0.05; **p < 0.01 (χ² test for expression and ANOVA; ANOVA for amplitude).