Figure 6.

Best1 current expressed in naive sensory neurons displays similar biophysical properties than CaCC recorded in axotomized neurons. A, The voltage relationships of Ca²⁺-activated current recorded in axotomized neurons (a) or in Best1-transfected naive neurons (b) were obtained by subtracting control ramp current from A23187-activated current. Both voltage relationships measured at steady amplitude are linear. By partial substitution with methane sulfonate (30 mm CsCl/115 mm Cs-methane sulfonate) for intracellular chloride ions, reversal potentials were shifted from −2 mV to approximately −30 mV, which indicates Cl⁻ conductance (c). B, Current traces of CaCC expressed in axotomized neurons (a) and of Best1 current expressed in naive neurons (b), recorded with 500 ms depolarizing pulses from −80 to +60 mV in 10 mV increments, show no time dependence.