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. 2000 Aug 15;97(18):9896–9901. doi: 10.1073/pnas.160266197

Figure 2.

Figure 2

(A–D) Northern blot of kidney mRNA of normal mouse, rat, and human, hybridized with mouse (A) and human (B) cDNA probes. A single transcript of ≈1.5 kb is observed in all of the species (A and B). Loading of equal amount of RNAs of various species is reflected by the similar intensity of 28S and 18S bands in the blot stained with methylene blue (C) and by comparable β-actin expression (D). (E–J) Southern blots of genomic DNA of mouse, rat, and human, digested with restriction endonucleases and hybridized with 5′ end-specific (E, G, and I) and 3′ end-specific (F, H, and J) RSOR cDNA probes. The banding profile is variable, suggesting that the genomic organization of the RSOR is different among the three species.