Figure 6.

(A) Titration curve depicting a high affinity of the RSOR fusion protein for NADPH. (B) SDS/PAGE of various proteins analyzed by Western blot hybridized with antifusion protein antibody. Lane 1, ALR1; lane 2, ALR2; lane 3, kidney cortex extract; lane 4, RBG column eluant of the kidney extract, lane 5, RSOR fusion protein; lane 6, BSA. Reactivity with the eluant from RBG column (lane 4) indicates recognition of NADPH binding protein by the anti-RSOR antibody. Mild cross-reactivity with the ALR1 also is observed (lane 1). (C) Comparison of amino acid sequence of AKR motifs. Motif sequences are boxed. The NADPH binding sites are bolded and underscored. M-RSOR*, mouse RSOR; M-17βHSD, mouse 17β-hydroxysteroid dehydrogenase; B-PGFS, bovine lung prostaglandin F synthase; H-BABPDD, bile acid binding protein dihydrodiol dehydrogenase; Frog-Rho, ρ-crystallin; R-ALR1, rat ALR1; M-ALR2, mouse ALR2.