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. Author manuscript; available in PMC: 2009 Oct 14.
Published in final edited form as: J Immunol. 2007 Oct 15;179(8):5146–5158. doi: 10.4049/jimmunol.179.8.5146

FIGURE 10.

FIGURE 10

The CD4+CD25high T cells require cell:cell contact and not IL-10 or TGF-β to exert their suppressive activity. a, Cytokine neutralization. A total of 5.0 × 105 p161–180-primed LN cells (responders) were cultured alone or cocultured with 5.0 × 105 CD4+CD25high T cells with 125 μg/ml p161–180 as stimulant in the presence or absence of 20 μg/ml anti-IL-10 or anti-TGF-β. Data are presented as cpm ± SE of triplicate cultures (*, p < 0.02; **, p < 0.001; ***, p < 0.039). Shown is a representative experiment of two. b, Cell:cell contact dependency. A total of 5 × 105 or 2.5 × 105 CD4+CD25high T cells were seeded alone or cocultured with 5 × 105 p161–180-primed LN cells (responders) with p161–180 Ag (phase 1). After 48 h, supernatant from each culture condition was added to fresh p161–180 responder cells with peptide stimulant (phase 2). Additional wells from phase 1 and all phase 2 cultures were assayed for proliferation by [3H]thymidine uptake. Data are presented as cpm ± SE of triplicate cultures. Shown are results from two combined experiments.