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. Author manuscript; available in PMC: 2009 Nov 28.
Published in final edited form as: Nature. 2009 Apr 22;459(7246):596–600. doi: 10.1038/nature08030

Figure 1. Tissue and subcellular expression of mammalian TPC2.

Figure 1

a, Hydropathy plot of human TPC2. Window size = 9 a.a. b, Evolutionary relationships of TPCs with single-pore and four-pore domain channels. h, human; d, dog; r, rat; at, Arabidopsis thaliana. c, Northern blot analysis of TPC2 expression in human tissues. d, Colocalization of endogenous TPC2 with LAMP-2 in untransfected HEK293 cells. e and f, Colocalization of HA-hTPC2 with LAMP-2 (e) and LysoTracker (f) in hTPC2 stable cell line. Right panel in f shows stacked image of overall LysoTracker accumulation relative to expressed HA-hTPC2 (3D projections, supplementary Movie 1). Scale bars = 10 μm.