Figure 3.

Northern hybridization showing the time kinetics of siRNAs accumulation in tomato plants agroinfiltrated with VA/pCAMBIA1391Z. Northern hybridization using CR-AC3 DNA fragment as a probe showing the formation of siRNAs in infiltrated tomato leaves at indicated time points (dpi). A labeled 21-mer oligonucleotide was used as a size marker. (B) The relative intensities of the siRNA bands were estimated and the normalized values with respect to corresponding loading controls are plotted as bar graphs. The value at 7 dpi was arbitrarily assigned as 100%. The standard deviations based on three independent experiments are shown.