Table 2.
Reference gene primer sequences and amplicon characteristics.
| Gene symbol | Forward primer sequence [5'-3'] | Reverse primer sequence [5'-3'] | Positions in cDNA | Amplicon length (bp) | Tm (°) | PCR efficiency (%) | Regression coefficient (R2) |
| ACT11 | ATCTTGACTGAGCGTGGTTATTCC | GCTGGTCCTGGCTGTCTCC | Exon3/Exon3 | 126 | 83.3 | 104 | 0.998 |
| ACT2/7 | AATTCACGAGACCACCTACAAC | TGAGCCACCACTAAGAACAATG | Exon3/Exon3 | 91 | 78.8 | 98 | 0.999 |
| CYP | ACGACGAAGACGGAGTGG | CGACGACGACAGGCTTGG | Exon | 130 | 87.8 | 96 | 0.999 |
| EF1b | CCACTGCTGAAGAAGATGATGATG | AAGGACAGAAGACTTGCCACTC | Exon4/Exon5 | 134 | 82.0 | 94 | 0.998 |
| TUA5 | TGCCACCATCAAGACTAAGAGG | ACCACCAGGAACAACAGAAGG | Exon6/Exon7 | 103 | 81.0 | 104 | 0.999 |
| TUB4 | GGCGTCCACATTCATTGGA | CCGGTGTACCAATGCAAGAA | Exon2/Exon2 | 111 | 83.8 | 106 | 0.999 |
| UBQ10 | TCCCACCAGACCAGCAGAG | CACGAAGACGCAACACAAGG | Exon | 117 | 84.0 | 98 | 0.999 |
| HDC | AGGTCGTTGTTGTCTCAGGTG | CGTGCCGCTTCAGTCTCAG | Exon6/Exon6 | 88 | 80.0 | 95 | 0.999 |
| SKIP16 | GAGCCCAAGACATTGCGAGAG | CGGAAGCGGAAGAACTGAACC | Exon1/Exon1 | 60 | 80.8 | 102 | 0.999 |
| MTP | CGCTCCAAGTGCTCCTCATTAG | TGAAGTAACCGACGCCAACG | Exon1/Exon2 | 71 | 82.8 | 93 | 0.999 |
| PEPKR1 | AGCAACCAAACAAATCCTGAACAAC | CCAACATCCAACTCTCCACAACC | Exon6/Exon6 | 68 | 75.6 | 98 | 0.995 |
| TIP41 | AGGATGAACTCGCTGATAATGG | CAGAAACGCAACAGAAGAAACC | Exon5/Exon6 | 88 | 77.8 | 105 | 0.997 |
| UKN1 | TGGTGCTGCCGCTATTTACTG | GGTGGAAGGAACTGCTAACAATC | Exon1/Exon1 | 74 | 78.3 | 96 | 0.994 |
| UKN2 | GCCTCTGGATACCTGCTCAAG | ACCTCCTCCTCAAACTCCTCTG | Exon5/Exon6 | 79 | 79.5 | 93 | 0.999 |