Table 1.
Oligonucleotide primers
| Gene Symbola | Primer Pair (5 ′ to 3 ′) | Product Size (bp) |
|---|---|---|
| For qualitative and real-time RT-PCR | ||
| Actb | GGCTGTATTCCCCTCCATCG | 154 |
| CCAGTTGGTAACAATGCCATGT | ||
| Fzd1 | GCGACGTACTGAGCGGAGTG | 150 |
| TGATGGTGCGGATGCGGAAG | ||
| Fzd2 | CTCAAGGTGCCGTCCTATCTCAG | 156 |
| GCAGCACAACACCGACCATG | ||
| Fzd3 | GGTGTCCCGTGGCCTGAAG | 194 |
| ACGTGCAGAAAGGAATAGCCAAG | ||
| Fzd4 | GACAACTTTCACGCCGCTCATC | 181 |
| CCAGGCAAACCCAAATTCTCTCAG | ||
| Fzd5 | AAGCTGCCTTCGGATGACTA | 129 |
| TGCACAAGTTGCTGAACTCC | ||
| Fzd6 | TGTTGGTATCTCTGCGGTCTTCTG | 110 |
| CTCGGCGGCTCTCACTGATG | ||
| Fzd7 | ATATCGCCTACAACCAGACCATCC | 193 |
| AAGGAACGGCACGGAGGAATG | ||
| Fzd8 | GTTCAGTCATCAAGCAGCAAGGAG | 122 |
| AAGGCAGGCGACAACGACG | ||
| Fzd9 | ATGAAGACGGGAGGCACCAATAC | 107 |
| TAGCAGACAATGACGCAGGTGG | ||
| Fzd10 | ATCGGCACTTCCTTCATCCTGTC | 199 |
| TCTTCCAGTAGTCCATGTTGAG | ||
| Gapdh | CCCCAATGTGTCCGTCGTG | 84 |
| GCCTGCTTCACCACCTTCT | ||
| Ncam1 | CCCAGCCAAGGAGAAATCAG | 123 |
| CTGGTTTGGGCTCAGCTTCT | ||
| Ntrk2 | CTGGGGCTTATGCCTGCTG | 100 |
| AGGCTCAGTACACCAAATCCTA | ||
| Ryk | GGTCTTGATGCAGAGCTTTACT | 170 |
| CCCATAGCCACAAAGTTGTCTAC | ||
| Tbp | CCCCACAACTCTTCCATTCT | 103 |
| GCAGGAGTGATAGGGGTCAT | ||
| For cloning partial cDNAs as riboprobe templates | ||
| Fzd1 | CAGGTTCTGCAAAAGCTTCC | 682 |
| TCGGTTACTGCACTCCCTCT | ||
| Fzd2 | TTTAAAAGCTGCCCTGTGCT | 642 |
| CTACCGGGAGAGAAGGGAAC | ||
| Fzd3 | TTTGGGTTGGAAGCAAAAAG | 616 |
| GACACTCTGCCCAAGAAAGC | ||
| Fzd4 | AATTCTAGGCAGCCCCTGTT | 658 |
| CCAGCATTCTGGAGGTTCAT | ||
| Fzd5 | CACTCAAGACTCCGGAGAGG | 607 |
| TCCTGGGAGTGTAGGTTTGG | ||
| Fzd6 | TTGCTAGCCCTGACTGTCCT | 613 |
| CTCCTTTTGGGGAAGGTAGG | ||
| Fzd7 | TTTCAAGAGGAGGCCAAGAA | 609 |
| CCCTGTCTGGAGGAAAAACA | ||
| Fzd8 | TGGCAGGACATGAGAAAGTG | 682 |
| CGGTTGTGCTGCTCATAGAA | ||
| Fzd9 | AGTTTCCTCCTGACCGGTTT | 692 |
| CAAGGCCCTGAGCTTTACTG | ||
| Fzd10 | CTTTGCTGCCTGTGCATAAA | 616 |
| CAATAAGCCCTCTGGTGCTC | ||
| Ryk | GAACGACTTGCGAAGTGTCA | 700 |
| CAGAGTCATGAGCTCCCACA | ||
a
Actb, actin, beta; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Ncam1, neural cell adhesion molecule 1; Ntrk2, neurotrophic tyrosine kinase, receptor, type 2; Tbp, TATA box binding protein.