Table 1.
Wild-Type | klp61f | [WT]g | [3YF]h | |
---|---|---|---|---|
Interior Divisions (Cycles 1–9) | n=201 | n=532 | n=287 | n=410 |
Monastrala | 0.5 | 1.88 | 0 | 0 |
Anastralb | 0.5 | 4.89 * | 0.7 * | 0 |
Promiscious MTsc | 0 | 0.75 | 0.35 | 0.49 |
Multi MTOCd | 1.99 | 3.76 | 5.57 | 11.75 * |
Monopolare | 0 | 6.77 ** | 0.35 ** | 8.29 ** |
TOTAL | 2.99 | 18.05 | 6.97 | 20.53 |
Cortical Divisions (Cycles 11–13) | n=118 | n=104 | n=191 | n=175 |
Monopolar | 0.85 | 0 | 0.52 | 1.14 |
Promiscuous MTs (Meta) | 0 | 1.83 | 4.19 | 1.71 |
Promiscuous MTs (Ana) | 0 | 0 | 2.09 | 6.86 ^ |
TOTAL | 0.85 | 1.83 | 7.78 | 9.71 |
TOTAL DEFECTS | 3.84 | 19.88 | 14.75 | 30.24 |
n represents the number of spindles assayed from 30 (interior) or 3 (cortical) embryos
bipolar spindles with only one centrosome
bipolar spindles with no centrosomes
microtubule spurs or interactions between spindles
bipolar spindles with multiple MTOCs
monopolar spindles
statistically compared to wild type embryos
statistically compared to klp61f embryos
statistically compared to [WT] embryos
MT = microtubules
0.001>p
0.01>p
0.05>p