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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Leukemia. 2009 Jun 4;23(10):1933–1935. doi: 10.1038/leu.2009.118

Figure 3.

Figure 3

The gene encoding Egr-1 is a direct target of Src family kinases (SFKs). (a) NB4 cells were treated for 1 h with vehicle alone, 10 µm PP2, 10 µm cycloheximide (CHX) or cycloheximide plus PP2. After the preparation of total RNA, reverse transcription-polymerase chain reaction (RT-PCR) was carried out using primers specific for Egr-1 mRNA (forward primer: 5′-AGCCCTACGAGCACCTGAC-3′; reverse primer: 5′-TGGGTTGGTCATGCTCACTA-3′). The RT-PCR products were electrophoresed on a 2% agarose gel and stained with ethidium bromide. β-Actin was used as an internal control. (b) NB4 cells were treated as in (a), followed by immunoblotting for Egr-1 or β-actin. The experiment was performed three times with similar results.