Table 3. Experiment 2 Microinjection/DID Procedure.

Arrows indicate the order in which procedures were performed (top-to-bottom). Animals were bilaterally implanted with guide cannulae aimed at the pVTA. The following day stylets were removed and replaced to assure they remained patent. On days 3–4 stylets were again manipulated, animals were restrained for 60s, and were then immediately given access to ethanol using DID procedures. On days 5–6 animals underwent the same procedure except restraint was for 90s prior to DID access. On days 7–8 animals had mock microinjections and 90s restraint prior to DID. The final day (9) animals were microinjected with WIN or vehicle and given access to DID.

Days 1	Day 2	Days 3–4	Days 5–6	Days 7–8	Day 9
Surgery	Stylet Removal	Stylet Removal	Stylet Removal	Stylet Removal	Stylet Removal
	(Light)	(3hrs into Dark)	(3hrs into Dark)	(3hrs into Dark)	(3hrs into Dark)
				Mock Inj.	WIN Inj.
		60s Restraint	90s Restraint	90s Restraint	90s Restraint
		2hr DID	2hr DID	2hr DID	2hr DID