Skip to main content
. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Neuroscience. 2009 Aug 7;164(2):424–434. doi: 10.1016/j.neuroscience.2009.08.007

Table 4. Experiment 2 Microinjection/Locomotor activity Procedure.

Arrows indicate the order in which procedures were performed (top-to-bottom). Animals were bilaterally implanted with guide cannulae aimed at the pVTA and allowed to rest for 5 days during which stylets were removed and replaced daily. On day 6 stylets were removed animals were restrained for 90s and were immediately placed in the activity monitors for 2 hours. On days 7–8 animals were given mock microinjections and immediately placed in the activity monitors for 2 hours. On the 9th day animals were given microinjections of WIN or vehicle after which they were monitored for 2 hours.

Days 1 Day 2–5 Day 6 Days 7–8 Days 9
Surgery Stylet Removal Stylet Removal Stylet Removal Stylet Removal
(Light) (3hrs into Dark) (3hrs into Dark) (3hrs into Dark)
90s Restraint Mock Inj. (90s) WIN Inj. (90s)
Activity Monitor Activity Monitor Activity Monitor