Figure 3.
Gr33a mutant flies display reduced avoidance to most bitter chemicals. (AG) The two-way choice behavioral assays were performed by giving the flies a choice between 1 mM sucrose alone or 5 mM sucrose in combination with the indicated concentrations of aversive chemicals. A value of 1 indicates a complete preference for 1 mM sucrose, while a value of 0 indicates a complete preference for the 5 mM sucrose mixed with the aversive chemical. A value of 0.5 indicates no preference for the two alternative tastants. The strains used were: Gr33a+ (w1118; blue lines), Gr33a1 (red), Gr33aGAL4 (green) and Gr33a1/Gr33aGAL4;UAS-Gr33a (black). We used one-way ANOVA followed by Scheffe's post-hoc analysis to perform tests for statistical significance. The error bars represent SEMs. The asterisks indicate statistically differences from the Gr33a+ control (* P<0.05, ** P<0.01). Detailed statistics are presented in Table S4.