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Examples of dendritic segments used in layer V spine density analyses as imaged from Control (A, D, G), Stress (B, E, H), and Rest (C, F, I) neurons, organized by layer I (A-C), layers II/III (D-F), and layer V (G-I). While no significant spine density differences were found per group, there were significant spine density differences across layers (layer I: 1.9 spines/μm, layers II/III: 3.8 spines/μm, layer V: 2.6 spines/μm). Scale bar, 5 μm.
