Figure 4. Compartments in nuclei isolated in 50% Ficoll, 100 µM K-Hepes by cell lysis with 0.5% Triton X-100 to permeabilise the nuclear membrane.

(A) proteins characteristic of different compartments visualised by immunofluorescence as described in Figure 3. Daxx is a further component of PML bodies. (B) GFP-coilin or GFP-PML isoform IV fusion proteins in nuclei of HeLa or U2OS cells, respectively. In the two left panels, approximate nuclear outlines were traced from overexposed images. Images are maximum intensity projections from deconvoluted confocal series. Bar 5 µm.