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ChIP analysis results revealed that TBX3 transcription factor regulates GATA3 and GLI2/3 target genes. PCR amplification on LA7 cells (panel A): input samples (total genome, lane 1), preimmune sera (PI, lane 2) and chromatin immunoprecipitated material (lane 3). The expected GLi3 (rat) or GLI3(human) bands were obtained in LA7 or BT474 cells (panels A and B). Beta actin (ACTB for human; Actb for rat) was used as control in both panels.
