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. Author manuscript; available in PMC: 2010 Jan 2.
Published in final edited form as: N Engl J Med. 2009 Jul 2;361(1):11–21. doi: 10.1056/NEJMoa0810457

Figure 1. Results of Western Blotting of Glomerular Proteins with Serum from Patients with Idiopathic Membranous Nephropathy.

Figure 1

The top of Panel A shows the results of Western blotting of extract of human glomerular proteins with serum samples from each of five patients with idiopathic membranous nephropathy (MN1 through MN5) and five patients with other proteinuric conditions (two with focal and segmental glomerulosclerosis [FGS1 and FGS2] and three with diabetic nephropathy [DN1, DN2, and DN3]). Serum samples from the five patients with membranous nephropathy all recognized a band of approximately 185 kD, whereas the samples from the patients with other diseases did not. The bottom of Panel A shows the results of Western blotting, with reactive serum samples from the five patients with membranous nephropathy, of glomerular proteins that were deglycosylated with peptide N-glycosidase F (PNGase F+) or not deglycosylated (PNGase F−). All five samples showed the 185-kD native antigen and a deglycosylated protein of approximately 145 kD. Panel B shows the specificity of the reactivity of serum samples to the 185-kD antigen.