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. 2009 Oct 5;187(1):33–42. doi: 10.1083/jcb.200903003

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© 2009 Li et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — OMA-1 interacts with SPN-2 and the mei-1 3′ UTR. (A) DIC image of a metaphase embryo with skewed spindle (arrowheads mark the centrosomes), and confocal images of embryos stained for MEI-1 (green) and DAPI (blue). Bar, 10 µm. (B and C) In vitro pull-down assays using the proteins shown; eluates were analyzed by Western blotting (WB) with the indicated antibodies. The asterisk in B indicates a band of aggregated MBP in the MBP input lane. (D) MBP–OMA-1 or MBP was incubated with biotinylated sense or antisense RNA from the mei-1 3′ UTR. RNA-bound proteins were analyzed by Western blotting.