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. 2009 Oct 5;187(1):135–148. doi: 10.1083/jcb.200905103

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© 2009 Pigino et al.

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Figure 2. — TEM micrographs of in situ IFT trains in flat-embedded flagella from WT and fla14 mutant cells of C. reinhardtii. WT flagella display two types of trains, each with a characteristic length and ultrastructure. (a–d) Four short IFT trains (framed in red lines). (e) A magnified view of panel d showing that these trains are electron dense and compact, with an internal 16-nm repeat of small “lollipop-like particles.” (f–i) Four long IFT trains (framed in red lines). At higher magnification (l), these trains are less compact than short trains and are characterized by an arc-shaped pattern, not seen in short trains. (m, n, and o) Three long IFT trains from fla14 mutant cells, which lack retrograde IFT. These trains are characterized by a structure and a repeat very similar to those observed in long trains from WT cells.