Table 1.
Rate constants for transferrin recycling in transfected TRVb cells loaded with the marker at either 37°C or 16°C
| Transfected plasmid | Kr(37°C ➞ 37°C) min−1 | Kr(16°C ➞ 37°C) min−1 |
|---|---|---|
| Vector | 0.058 ± 0.007 | 0.060 ± 0.001 |
| Rab11-WT | 0.036 ± 0.001 | 0.049 ± 0.006 |
| Rab11-Q70L | 0.035 ± 0.002 | 0.053 ± 0.001 |
| Rab11-S25N | 0.024 ± 0.002 | 0.027 ± 0.001 |
| Rab11-ΔC | 0.052 ± 0.004 | 0.051 |
First order rate constants (Kr) for transferrin recycling were determined from the data in Figs. 2 and 4 and are presented as the means ± SE of two separate experiments. The middle column gives the recycling rate constants at 37°C for transferrin molecules interiorized at 37°C [Kr (37°C ➞ 37°C)], and the rightmost column gives the rate constants for molecules interiorized at 16°C [Kr(16°C ➞ 37°C)].