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. 2009 Oct 5;187(1):81–89. doi: 10.1083/jcb.200812084

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© 2009 Engel et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Quantitative photobleaching confirms that IFT trains in short flagella contain more KAP protein. (A) At different time points after pH shock, KAP-GFP cells were for fixed and photobleached under constant TIRF illumination. The mean intensity was measured from 0.4 × 0.4 µm ROIs (red squares) centered on IFT trains. Bar, 2 µm. (B) Three examples of intensity plots of ROIs from flagella of different lengths. The raw data were filtered with a Chung-Kennedy edge-preserving algorithm to enhance the detection of stepwise GFP bleaching events. (C) Histograms of the number of KAP-GFP proteins measured in ROIs from long, mid-length, and short flagella (yellow vertical bars denote the mean, yellow horizontal bars show the standard deviation). n = 230 ROIs from 90 flagella.