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. 2009 Oct 5;187(1):43–51. doi: 10.1083/jcb.200903076

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© 2009 O'Connell et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Chromatin in Nuf2-depleted cells attracts astral microtubules. (A–C) HeLa cells treated with HU were either mock transfected (A) or transfected with siRNA specific for human Nuf2 (hNuf2; B and C). After MUG entry, cells were fixed and stained for chromatin, Hec1, or α-tubulin. (D) Normal mitotic HeLa cell expressing centrin1-GFP (arrowheads) depleted of Nuf2. Bars, 5 µm.