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. 2009 Oct 15;20(20):4362–4370. doi: 10.1091/mbc.E09-07-0539

Figure 2.

Figure 2.

TβRIII associates with ALK6 and ALK3. COS-7 cells were transiently transfected with 1.5 μg of either vector, wild-type HA-tagged TβRIII, or a mutant HA-TβRIII construct (HA-TβRIII-ΔGAG, HA-TβRIII-ΔcytoΔGAG, and TβRIII-T841A-ΔGAG) along with 1.5 μg of HA-ALK3 or HA-ALK6, as indicated. (A) The lysates were immunoprecipitated with TβRIII-specific antibody that recognizes the extracellular domain of TβRIII, resolved by SDS-PAGE, and immunoblotted with ALK6-specific antibody to detect coprecipitated ALK6 (top; 1:1500 dilution of ALK6 primary antibody), TβRIII antibody for TβRIII (middle; 1:5000 dilution of TβRIII primary antibody), and ALK6-specific antibody for ALK6 expression in the lysate (bottom; 1/20 input of total lysate). (B) The lysates were immunoprecipitated with TβRIII-specific antibody and resolved by SDS-PAGE and immunoblotted with ALK3-specific antibody to detect coprecipitated ALK3 (top; 1:1500 dilution of ALK3 primary antibody), TβRIII antibody for TβRIII (middle; 1:5000 dilution of TβRIII primary antibody), and ALK3 antibody for ALK3 expression in the lysate (bottom; 1/20 of total lysate).