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The DLC protein family contains a conserved polybasic region and interacts with PI(4,5)P2. (A) Sequence comparison of DLC1 from different species and human DLC2 and DLC3. Basic amino acids are highlighted in bold. (B) GFP-tagged DLC1, DLC2 and DLC3 were tested for their ability to bind to immobilized PI(4,5)P2 as described in Figure 1. A representative experiment with triplicate samples is shown. Error bars, SEM.
