Figure 3.

Pob1 is a cdc42-879 suppressor. (A) Growth of wild-type (wt) and cdc42-879 transformed with the plasmid pREP42HA empty or carrying pob1⁺ or pob1-664 as inserts. Cells were spotted at one-fourth dilution in minimal medium and incubated at 28 or 36°C during 3 d. (B) Fluorescence images of wt and cdc42-879 cells transformed with the plasmid pAU or pAU-pob1⁺. Cells grown at 28°C and shifted to 36°C for 5 h were stained with calcofluor (CF) (top) or AlexaFluor 488-phalloidin (bottom). Fluorescence images of Myo52-GFP (C) or For3-3GFP (D) in the same strains grown at 28°C. Bar, 5 μm.