Figure 5.

Cdc42 regulates Pob1 localization. (A) Localization of GFP-Pob1 in wild-type (wt), cdc42-879, cdc42D76G, cdc42L160S, and cdc42-879 cells transformed with the overexpression plasmid pREP41Xcdc42⁺ grown at 28°C. (B) Total Pob1-myc in lysates from wild-type and cdc42-879 cells grown at 28°C visualized by Western blot by using anti-myc antibody (top). Tubulin was visualized in the same extracts as loading control (bottom). (C) Two-hybrid analysis of the interaction between Pob1 or Scd2 (pAS2) with Cdc42 or Cdc42–879 (pACT2). Interaction was assessed by growth on YNB plates without histidine and supplemented with 40 mM 3-amino triazol (3-AT).