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. 2009 Oct 15;20(20):4390–4399. doi: 10.1091/mbc.E09-03-0207

Figure 7.

Figure 7.

The N-terminal region of Pob1 is required for For3 localization. (A) Wild-type and pob1ΔN strains grown at 30°C in YES expressing either for3-3GFP or for3DAD*-2GFP stained with calcofluor (left) and with AlexaFluor 488-phalloidin (middle) to see the actin. Fluorescence images of For3-3GFP or For3-DAD*-2GFP in the same strains grown at 30°C in YES (right). (B) for3DAD* expression improves the thermosensitive slow growth of pob1ΔN cells. Cells spotted at 1/4 dilution in YES medium and incubated at 32, 34, 35, and 36°C for 3 d. (C) Localization of GFP-For3N (including For3 amino acids 1–702) expressed from a plasmid under control of the medial-strength nmt1 promoter in wild-type and pob1ΔN cells.