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The SAM domain of Pob1 is required for For3 localization. (A) Fluorescent microscopy of GFP-pob1ΔSAM–expressing cells. Bar, 5 μm. (B) Actin staining of pob1ΔSAM endogenously expressing for3+-3GFP or for3DAD*-2GFP alleles (top). Localization of For3-3GFP or For3DAD*-2GFP in pob1ΔSAM (bottom). (C) for3DAD* expression improves the thermosensitive slow growth of HA-pob1ΔSAM cells. Cells spotted at one-fourth dilution in YES medium and incubated at 32, 34, 35, and 36°C for 3 d.
