Figure 1.

Disruption of the UCE gene by insertional mutagenesis. A β-galactosidase-Neomycin cassette (B-Geo) was inserted between exons 4 and 8 of the gene encoding UCE (Nagpa) in mouse, by using a targeting mutagenesis approach. The localization of the probes (5′- and 3′-external) and restriction sites (XhoI-BglII) used for the Southern blot experiment (see Figure 2A), and the primers used for genotyping the mice (see Figure 2B) are shown.