Figure 6.

Localization of Vamp7B on contractile vacuoles in living cells. Top row, Images of living wild-type cells in water expressing GFP-Vamp7B. Vamp7B is found on contractile vacuoles as they expand (arrowhead) and as they contract (arrow). See Supplemental Movies S6 and S7. Bottom row, Images of living AP2α/AP180–double null cells in water expressing GFP-Vamp7B. An entire cycle of a contractile vacuole is labeled with GFP-Vamp7B, from expansion to contraction (arrow). The end of a contraction phase of a second contractile vacuole is also labeled with GFP-Vamp7B (arrowhead). Scale bar, 10 μm. See Supplemental Movies S8 and S9.