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The SNARE protein Vti1 localizes equivalently on the contractile vacuoles of wild-type and mutant cells. Wild-type, AP2α−, AP180−, and AP2α/AP180 DKO cells expressing GFP-Vti1 (green) were immunostained with the contractile vacuole marker Rh50 (red). In all four cell lines, GFP-Vti1 labels contractile vacuoles (arrows). Scale bar, 10 μm.
