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Quantitative RT–PCR analysis of HMLα1, HMLα2 and HMLα3 mRNA in wild-type (CK213), sir2Δ (SAY569), sir4Δ (LRY2038), sum1Δ (LRY2035), rfm1Δ (LRY2528), and sir2Δ sum1Δ (LRY2533) strains. The amount of cDNA was first normalized to the control locus ACT1. The values shown here represent the relative amount of cDNA for each deletion strain compared to the wild-type strain. Error bars represent the SEM.
