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. 2009 Oct 13;2009:952304. doi: 10.1155/2009/952304

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Copyright © 2009 M. S. Krasnikova et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Agarose gel analysis of PCR products. (a) Amplification of genomic DNA sequences flanked by miR390 sites. PCR products were obtained on Nicotiana tabacum genomic DNA with primers P-Tas3 and M-Tas3/caa (Lane 1), on N. tabacum genomic DNA with primers P-Tas3 and M-Tas3/aca (Lane 2), on Arabidopsis thaliana genomic DNA with primers P-Tas3 and M-Tas3/caa (Lane 3), and on A. thaliana genomic DNA with primers P-Tas3 and M-Tas3/aca (Lane 4). Arrow points to the 170-nt PCR product. M is DNA size markers. Marker fragment sizes in base pairs are indicated on the left. (b) Amplification of N. tabacum genomic DNA and cDNA preparation. PCR products were obtained on N. tabacum genomic DNA with primers P-Tas3 and M-Tas3/caa (Lane 1), on N. tabacum cDNA with primers P-Tas3 and M-Tas3/caa (Lane 2), and on N. tabacum cDNA with primers P-Tas3 and M-Tas3/aca (Lane 3). M is DNA size markers. Marker fragment sizes in base pairs are indicated on the left.