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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Development. 2008 Apr 16;135(10):1853–1862. doi: 10.1242/dev.015297

Figure 7.

Figure 7

Characterization of hematopoietic precursors on the pronephric tubules. (A) FITC was uncaged in 5 CD41:eGFP+ cells (arrows) at 4 dpf along the left pronephric tubule. (B) Animals were fixed three days later and analyzed for uncaged FITC. Ductal cells migrated from where they were targeted on the left pronephric tubule (green arrowheads, left panel) to the left anterior pronephros (red arrowhead). Contralateral anterior pronephri were not colonized (right panel). (C) By 65 hpf, AGM expression of the c-myb:eGFP transgene becomes restricted to the pronephric tubules. c-myb:eGFP+ tubular cells (T) were purified away from GFP+ neural cells (N) in dissected trunks by flow cytometry and analyzed for hematopoietic gene expression (right panel).