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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Curr Biol. 2009 Sep 24;19(19):1599–1607. doi: 10.1016/j.cub.2009.08.045

Figure 3. cil-1 regulates PI(3,5)P2 and PI(3,4,5)P3 subcellular distribution.

Figure 3

GFP-tagged PI specific markers in the intestine of adult males are 2×FYVE for PI(3)P, AKT(PH domain) for PI(3,4,5)P3 and PI(3,4)P2, and PLC-delta (PH domain) for PI(4,5)P2. (A-C) In the WT intestine: (A) PI(3)P labels mesh-like, tubulovesicular structures in the cytoplasm without obvious PM labeling. (B) PI(3,4,5)P3 and PI(3,4)P2 label similar tubulovesicular structures as well as the apical (arrowheads) and basolateral (arrow) PM. (C) PI(4,5)P2 predominantly labels the apical (arrowheads) in addition to the basolateral (arrow) PM. (D-F) In the cil-1(my15) intestine: (D) PI(3)P appears soluble in the cytoplasm. (E) PI(3,4,5)P3 and PI(3,4)P2 lose their tubulovesicular pattern, appearing diffuse in the cytoplasm. The PM labeling is less prominent in cil-1 mutants (arrowheads). (F) PI(4,5)P2 remains enriched in the PM. (G-H) tdTomato-tagged 2×FYVE domain (PI(3)P marker) expression in male specific neurons of WT and cil-1(my15). (G) In WT CEMs, the PI(3)P marker is bright in the nuclei (denoted as N), small puncta in the cell bodies, but almost absent from cilia (blank yellow arrowhead). (G′) Similarly, in WT RnBs, the PI(3)P marker is confined to cell bodies (nuclei and small puncta). (H) In cil-1(my15) CEMs, the PI(3)P marker is visible in cilia and dendrites (arrows) in addition to cell bodies. The inset shows PI(3)P marker labeling ciliary and dendritic regions. (H′) In cil-1(my15) RnBs, the PI(3)P marker is occasionally visible in cilia (yellow arrowhead) in addition to cell bodies. Scale bar, 10um.