Figure 3. Nucleostemin expression is increased by pathological stress.

(A) Confocal microscopy showing increased nucleostemin immunoreactivity (green, at arrows) in myocardial sections from tropomodulin overexpressing transgenic (TOT) mice suffering from chronic dilated cardiomyopathy. Tropomyosin (red) labels sarcomeric structure and nuclei are labeled with Topro-3 stain (blue). Single channel scans that were used for creation of the color overlays are shown on the left of each panel. (B) Immunoblot and quantitation of nucleostemin protein expression in lysates prepared from nontransgenic (NTG) or TOT hearts show a significant increase in protein associated with the heart failure phenotype. (C) Confocal microscopy showing increased nucleostemin immunoreactivity (green, at arrows) proximal to a large vessel in myocardial sections from mice subjected to pressure overload hypertrophy by trans-aortic constriction at four days after banding.