Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Aug;92(2):311–317. doi: 10.1128/jb.92.2.311-317.1966

Genetic Differentiation by Nucleic Acid Homology II. Genotypic Variations Within Two Mycoplasma Species

Norman L Somerson a,1, Paul R Reich a, Barbara E Walls a, Robert M Chanock a, Sherman M Weissman a
PMCID: PMC276240  PMID: 16562112

Abstract

Somerson, Norman L. (National Institutes of Health, Bethesda, Md.), Paul R. Reich, Barbara E. Walls, Robert M. Chanock, and Sherman M. Weissman. Genetic differentiation by nucleic acid homology. II. Genotypic variations within two Mycoplasma species. J. Bacteriol. 92:311-317. 1966.—A deoxyribonucleic-ribonucleic acid (DNA-RNA) homology technique was used to determine genetic relatedness among the nucleic acids of eight mycoplasmas which were serologically classified as Mycoplasma hominis type 1. The DNA preparations from these organisms were each found to be distinct. No subgrouping of the M. hominis type 1 strains could be demonstrated. In contrast, when the nucleic acids from six serologically related mycoplasmas which were isolated from tissue cultures were studied, the DNA from these species could not be distinguished. The DNA buoyant densities of the tissue culture isolates were similar. These isolates were closely related genetically to a porcine mycoplasma, M. hyorhinis.

Full text

PDF
314

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARMSTRONG D., HENLE G., SOMERSON N. L., HAYFLICK L. CYTOPATHOGENIC MYCOPLASMAS ASSOCIATED WITH TWO HUMAN TUMORS. I. ISOLATION AND BIOLOGICAL ASPECTS. J Bacteriol. 1965 Aug;90:418–424. doi: 10.1128/jb.90.2.418-424.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BUTLER M., LEACH R. H. A MYCOPLASMA WHICH INDUCES ACIDITY AND CYTOPATHIC EFFECT IN TISSUE CULTURE. J Gen Microbiol. 1964 Feb;34:285–294. doi: 10.1099/00221287-34-2-285. [DOI] [PubMed] [Google Scholar]
  3. CARD D. H. PPLO of human genital origin; serological classification of strains and antibody distribution in man. Br J Vener Dis. 1959 Mar;35(1):27–34. doi: 10.1136/sti.35.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CLYDE W. A., Jr MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA. J Immunol. 1964 Jun;92:958–965. [PubMed] [Google Scholar]
  5. Girardi A. J., Hamparian V. V., Somerson N. L., Hayflick L. Mycoplasma isolates from primary cell cultures and human diploid cell strains. Proc Soc Exp Biol Med. 1965 Dec;120(3):760–770. doi: 10.3181/00379727-120-30649. [DOI] [PubMed] [Google Scholar]
  6. HERDERSCHEE D., RUYS A. C., VAN RHIJNG PLEUROPNEUMONIALIKE ORGANISMS IN TISSUE CULTURES. Antonie Van Leeuwenhoek. 1963;29:368–376. doi: 10.1007/BF02046089. [DOI] [PubMed] [Google Scholar]
  7. KRAEMER P. M. INTERACTION OF MYCOPLASMA (PPLO) AND MURINE LYMPHOMA CELL CULTURES: PREVENTION OF CELL LYSIS BY ARGININE. Proc Soc Exp Biol Med. 1964 Jan;115:206–212. doi: 10.3181/00379727-115-28871. [DOI] [PubMed] [Google Scholar]
  8. Leach R. H., Butler M. Compraison of mycoplasmas associated with human tumors, leukemia, and tissue cultures. J Bacteriol. 1966 Mar;91(3):934–941. doi: 10.1128/jb.91.3.934-941.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MUFSON M. A., LUDWIG W. M., PURCELL R. H., CATE T. R., TAYLOR-ROBINSON D., CHANOCK R. M. EXUDATIVE PHARYNGITIS FOLLOWING EXPERIMENTAL MYCOPLASMA HOMINIS: TYPE 1 INFECTION. JAMA. 1965 Jun 28;192:1146–1152. doi: 10.1001/jama.1965.03080260034010. [DOI] [PubMed] [Google Scholar]
  10. NICOL C. S., EDWARD D. G. Role of organisms of the pleuropneumonia group in human genital infections. Br J Vener Dis. 1953 Sep;29(3):141–150. doi: 10.1136/sti.29.3.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. OATES J. K., WHITTINGTON M. J., WILKINSON A. E. A note on the results of cultural and serological tests for pleuropneumonia-like organisms in Reiter's disease. Br J Vener Dis. 1959 Sep;35:184–186. doi: 10.1136/sti.35.3.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reich P. R., Somerson N. L., Hybner C. J., Chanock R. M., Weissman S. M. Genetic differentiation by nucleic Acid homology I. Relationships among Mycoplasma species of man. J Bacteriol. 1966 Aug;92(2):302–310. doi: 10.1128/jb.92.2.302-310.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reich P. R., Somerson N. L., Rose J. A., Weissman S. M. Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J Bacteriol. 1966 Jan;91(1):153–160. doi: 10.1128/jb.91.1.153-160.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rose J. A., Reich P. R., Weissman S. M. RNA production in adenovirus-infected KB cells. Virology. 1965 Dec;27(4):571–579. doi: 10.1016/0042-6822(65)90183-2. [DOI] [PubMed] [Google Scholar]
  15. TAYLOR-ROBINSON D., SOMERSON N. L., TURNER H. C., CHANOCK R. M. SEROLOGICAL RELATIONSHIPS AMONG HUMAN MYCOPLASMAS AS SHOWN BY COMPLEMENT-FIXATION AND GEL DIFFUSION. J Bacteriol. 1963 Jun;85:1261–1273. doi: 10.1128/jb.85.6.1261-1273.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tully J. G., Brown M. S., Sheagren J. N., Young V. M., Wolff S. M. Septicemia due to Mycoplasma hominis type 1. N Engl J Med. 1965 Sep 16;273(12):648–650. doi: 10.1056/NEJM196509162731207. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES