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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Nature. 2009 Jan 28;457(7231):882–886. doi: 10.1038/nature07684

Figure 2. OCLN silencing inhibits HCV entry.

Figure 2

Huh-7.5 (left) and Hep3B (right) cells transfected with irrelevant (grey), CD81 (checkered), or OCLN (black) specific siRNA pools were stained with CD81 (a and b) and OCLN (c and d) antibodies. Expression was analyzed by flow cytometry; the ratio between specific and isotype control staining is shown. Concurrently, siRNA-treated cells were challenged with HCVpp (e and f) and HCVcc (g). HCVpp infectivity was calculated as described in methods; HCVcc infection was quantified by the ratio of viral protein (NS5A) to isotype control staining. All samples are normalized to irrelevant-siRNA treatment. dMFI, difference in mean fluorescence intensity. Means and SD of at least triplicate experiments are shown.