Abstract
Dempsey, Walter B. (University of Florida, Gainesville). Synthesis of pyridoxine by a pyridoxal auxotroph of Escherichia coli. J. Bacteriol. 92:333–337. 1966.—A pyridoxal auxotroph of Escherichia coli B produced pyridoxol and pyridoxol 5′-phosphate during starvation for pyridoxal. The identification of these compounds was made both by bioassay and by ion-exchange chromatography. Pyridoxol 5′-phosphate oxidase activity was absent in extracts of the auxotroph. The rate of synthesis of total pyridoxine by a pyridoxal-starved culture of this auxotroph was 6.0 × 10−6 moles per mg per hr. Cellular content of pyridoxine was constant at 4.0 × 10−10 moles/mg.
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Selected References
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- DEMPSEY W. B. CONTROL OF PYRIDOXINE BIOSYNTHESIS IN ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:431–437. doi: 10.1128/jb.90.2.431-437.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dempsey W. B., Pachler P. F. Isolation and characterization of pyridoxine auxotrophs of Escherichia coli. J Bacteriol. 1966 Feb;91(2):642–645. doi: 10.1128/jb.91.2.642-645.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HENDERSON H. M. THE CONVERSION OF PYRIDOXINE PHOSPHATE INTO PYRIDOXAL PHOSPHATE IN ESCHERICHIA COLI. Biochem J. 1965 Jun;95:775–779. doi: 10.1042/bj0950775. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HURWITZ J. The enzymatic phosphorylation of pyridoxal. J Biol Chem. 1953 Dec;205(2):935–947. [PubMed] [Google Scholar]
- TURNER J. M., HAPPOLD F. C. Pyridoxamine phosphate-oxidase and pyridoxal phosphate-phosphatase activities in Escherichia coli. Biochem J. 1961 Feb;78:364–372. doi: 10.1042/bj0780364. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILSON A. C., PARDEE A. B. Regulation of flavin synthesis by Escherichia coli. J Gen Microbiol. 1962 Jun;28:283–303. doi: 10.1099/00221287-28-2-283. [DOI] [PubMed] [Google Scholar]