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. 2009 Oct 27;4(10):e7572. doi: 10.1371/journal.pone.0007572

Figure 5. Effect of the RBP-J deletion on the in vitro cultured EPC.

Figure 5

(A) The expression of CXCR4 on the cultured EPC was accessed by FACS analysis. (B, C) In vitro migration assay. Transwell culture was set up, with EPC from the RBP-J-deleted and the control mice in the upper chamber, and SDF-1α in the lower chamber. A photograph of EPC in the lower chamber (B) was taken, and cells in the lower chamber (C) were counted 18 h after the starting of the culture (bars = mean±SD; n = 4; ** P<0.01). (D) The formation of colonies by the cultured RBP-J knockout and the control EPC. (E) The number of total EPC formed 7 days after the culture. (F) The number of EPC attached on the gelatin-coated dishes 3 days after the culture. (bars = mean±SD; n = 4; * P<0.05).