Figure 2. Increased Meibomian gland size in Edar transgenic animals.

(A–C) Oil Red O stained eyelids of wild type, Edar^Tg951 and Edar^Tg951/Tg951 animals. Haematoxylin is used as a counterstain to reveal tissue structure. The sebaceous glands of the outer eyelid are above the green line in (A), while the Meibomian gland is below this line. (D) Quantification of Meibomian gland cross-sectional area on sampled slides for each genotype. The difference between wild type and Edar^Tg951 values did not achieve statistical significance in these analyses. Scale bar indicates 200 µm.