. Author manuscript; available in PMC: 2010 Nov 1.

Published in final edited form as: Biomaterials. 2009 Sep 13;30(33):6495–6513. doi: 10.1016/j.biomaterials.2009.08.016

Table 2.

Table 2A: Protein-Adsorption Kinetics from Binary Solution to Octyl Sepharose Particles (OS)

State 1

State 2

Protein Pairs

Condition

{(W_{B_{i}}^{o})}_{i, j}

(mg/mL)

{(W_{B_{j}}^{o})}_{i, j}

(mg mL)

(\frac{{(W_{B_{j}}^{o})}_{i, j}}{{(W_{B_{i}}^{o})}_{i, j}})

(D_i)_i,j mg/mL, (N)

(D_j)_i,j mg/mL, (N)

(\frac{{(D_{j})}_{i, j}}{{(D_{i})}_{i, j}})

(D_i)_i,j mg/mL, (N)

(\frac{{(D_{j})}_{i, j}}{{(D_{i})}_{i, j}})

Transition Period (min)

S_i,j, (N, R²)

HSA (i) + Fib (j)

Case 1

1.25

1.00

0.66 ± 0.05, (9)

0.46 ± 0.05, (9)

0.70 ± 0.09

0.53 ± 0.05, (3)

0.28 ± 0.03, (3)

0.53 ± 0.08

55–75

0.80 ± 0.02 (5, 0.99)

Case 2

2.00

3.00

1.50

0.98 ± 0.06, (9)

1.21 ± 0.08, (9)

1.23 ± 0.11

0.60 ± 0.01, (3)

0.74 ± 0.01, (3)

1.23 ± 0.03

45–80

Case 3

4.00

2.00

0.50

1.61 ± 0.09, (9)

0.60 ± 0.03, (9)

0.37 ± 0.02

1.11 ± 0.09, (6)

0.45 ± 0.04, (5)

0.41 ± 0.05

35–70

Case 4

2.00

6.50

3.25

0.96 ± 0.05, (9)

2.60 ± 0.11, (7)

2.71 ± 0.18

0.62 ± 0.05, (4)

2.01 ± 0.03, (4)

3.24 ± 0.26

50–75

Case 5

4.00

6.50

1.62

2.34 ± 0.07, (6)

2.86 ± 0.13, (5)

1.22 ± 0.26

1.57 ± 0.05, (5)

2.14 ± 0.04, (3)

1.36 ± 0.05

35–80

HSA (i) + IgG (j)

Case 1

1.25

1.00

0.56 ± 0.03, (9)

0.52 ± 0.03, (8)

0.94 ± 0.07

0.36 ± 0.02, (4)

0.35 ± 0.02, (3)

0.97 ± 0.08

40–80

0.88 ± 0.03 (5, 0.99)

Case 2

2.08

5.00

2.40

1.12 ± 0.06, (8)

2.20 ± 0.14, (8)

1.96 ± 0.16

0.90 ± 0.05, (4)

1.58 ± 0.11, (4)

1.76 ± 0.16

40–75

Case 3

4.17

5.00

1.20

2.21 ± 0.09, (6)

2.02 ± 0.16, (6)

0.91 ± 0.08

0.64 ± 0.10, (3)

0.75 ± 0.04, (3)

1.17 ± 0.19

25–80

Case 4

2.08

10.00

4.81

0.92 ± 0.04, (7)

4.04 ± 0.17, (7)

4.39 ± 0.26

0.69 ± 0.01, (4)

2.75 ± 0.08, (4)

3.98 ± 0.13

35–80

Case 5

4.17

10.00

2.40

2.26 ± 0.22, (9)

4.35 ± 0.15, (5)

1.92 ± 0.20

0.92 ± 0.14, (3)

2.10 ± 0.23, (4)

2.28 ± 0.43

50–85

HSA (i) + Ub (j)

Case 1

1.33

1.25

0.94

0.67 ± 0.02, (7)

0.66 ± 0.01, (9)

0.98 ± 0.03

0.55 ± 0.00, (3)

0.48 ± 0.01, (5)

0.87 ± 0.02

50–75

1.09 ± 0.04 (3, 0.98)

Case 3

5.00

2.08

0.42

2.29 ± 0.09, (9)

1.00 ± 0.06, (8)

0.44 ± 0.03

1.30 ± 0.03, (4)

0.60 ± 0.03, (5)

0.46 ± 0.02

40–75

Case 5

5.00

1.00

2.18 ± 0.29, (8)

2.49 ± 0.12, (5)

1.14 ± 0.16

0.96 ± 0.09, (4)

0.86 ± 0.10, (6)

0.90 ± 0.13

25–65

IgG (i) + Fib (j)

Case 1

3.00

1.50

0.50

1.22 ± 0.06, (13)

0.55 ± 0.03, (13)

0.45 ± 0.03

0.94 ± 0.04, (4)

0.41 ± 0.02, (4)

0.43 ± 0.03

65–80

0.90 ± 0.01 (3, 1.00)

Case 3

10.00

2.50

0.25

4.02 ± 0.37, (10)

0.97 ± 0.06, (10)

0.24 ± 0.03

2.60 ± 0.14, (3)

0.71 ± 0.01, (3)

0.27 ± 0.02

55–80

Case 5

10.00

7.00

0.70

4.89 ± 0.22, (9)

3.06 ± 0.14, (9)

0.63 ± 0.04

3.33 ± 0.22, (3)

2.27 ± 0.10, (3)

0.68 ± 0.05

45–80

FII (i) + Fib (j)

Case 1

1.50

1.25

0.83

0.74 ± 0.04, (11)

0.55 ± 0.03, (11)

0.74 ± 0.06

0.31 ± 0.03, (4)

0.25 ± 0.04, (4)

0.81 ± 0.15

65–75

0.96 ± 0.06 (3, 0.96)

Case 3

6.00

2.50

0.42

3.05 ± 0.18, (17)

1.04 ± 0.10, (17)

0.34 ± 0.11

No State 2

Case 5

6.00

7.00

1.17

2.35 ± 0.10, (10)

2.79 ± 0.11, (10)

1.19 ± 0.07

1.40 ± 0.19, (4)

1.86 ± 0.03, (4)

1.33 ± 0.18

50–75

FII (i) + IgG (j)

Case 1

1.50

1.00

0.64 ± 0.04, (18)

0.70 ± 0.04, (18)

1.09 ± 0.09

No State 2

0.97 ± 0.06 (3, 0.96)

Case 3

6.00

3.00

0.50

2.60 ± 0.11, (15)

1.17 ± 0.09, (17)

0.45 ± 0.04

No State 2

Case 5

6.00

10.00

1.67

2.46 ± 0.15, (8)

3.81 ± 0.20, (8)

1.55 ± 0.12

1.10 ± 0.08, (6)

2.01 ± 0.15, (6)

1.83 ± 0.19

40–65

Table 2B: Protein-Adsorption Kinetics from Binary Solution to Octadecyltrichlorosilane-Treated Glass Particles (OTS)

State 1

State 2

Protein Pairs

Condition

{(W_{B_{i}}^{o})}_{i, j}

(mg/mL)

{(W_{B_{j}}^{o})}_{i, j}

(mg/mL)

(\frac{{(W_{B_{j}}^{o})}_{i, j}}{{(W_{B_{i}}^{o})}_{i, j}})

(D_i)_i,j mg/mL, (N)

(D_j)_i,j mg/mL, (N)

(\frac{{(D_{j})}_{i, j}}{{(D_{i})}_{i, j}})

(D_i)_i,j mg/mL, (N)

(D_j)_i,j mg/mL, (N)

(\frac{{(D_{j})}_{i, j}}{{(D_{i})}_{i, j}})

Transition Period (min)

S_i,j, (N, R²)

HSA (i) + Fib (j)

Case 2

2.00

1.00

0.89 ± 0.18

0.10 ± 0.01, (3)

0.13 ± 0.01, (3)

1.30 ± 0.17

45–75

1.09 ± 0.06 (4, 0.95)

Case 4

2.00

4.00

2.00

0.35 ± 0.04, (8)

0.80 ± 0.07, (8)

2.28 ± 0.34

0.08 ± 0.02, (4)

0.13 ± 0.07, (4)

1.62± 0.96

45–70

Case 5

4.20

6.00

1.43

1.13 ± 0.09, (9)

1.77 ± 0.16, (9)

1.56 ± 0.19

0.18 ± 0.04, (3)

0.56 ± 0.01, (3)

3.11 ± 0.69

40–75

HSA (i) + IgG (j)

Case 2

2.00

4.00

2.00

0.51 ± 0.03, (11)

0.86 ± 0.07, (11)

1.68 ± 0.18

0.28 ± 0.05, (4)

0.44 ± 0.06, (4)

1.57 ± 0.36

60–80

0.91± 0.04 (4, 0.86)

Case 3

5.00

8.00

1.60

1.80 ± 0.09, (10)

2.81 ± 0.22, (10)

1.56 ± 0.14

0.91 ± 0.07, (5)

1.72 ± 0.03, (3)

1.89 ± 0.16

55–80

Case 5

5.00

12.00

2.40

1.76 ± 0.15, (10)

4.00 ± 0.50, (10)

2.27 ± 0.34

0.22 ± 0.05, (4)

0.79 ± 0.10, (3)

3.59 ± 0.95

55–80

Notes: See text for symbology and Section 3.2 for discussion of experimental conditions Cases 1–5.