Table 3.
Table 3A: Selectivity Factors for Reversed i,j Adsorption Competition to Octyl Sepharose Particles (OS) | |||
---|---|---|---|
Reversed Protein Pairs(from Table 2) | Experimental Condition | S̄i,j (N,R2) | Si,j (N,R2) |
HSA (j) + Fib (i) | Case 1 | 1.35 ± 0.03 (5, 0.99) | 0.80 ± 0.02 (5, 0.99) |
Case 2 | |||
Case 3 | |||
Case 4 | |||
Case 5 | |||
HSA (j) + IgG (i) | Case 1 | 1.18 ± 0.06 (5, 0.95) | 0.88 ± 0.03 (5, 0.99) |
Case 2 | |||
Case 3 | |||
Case 4 | |||
Case 5 | |||
HSA (j) + Ub (i) | Case 1 | 0.94 ± 0.02 (3, 1.00) | 1.09 ± 0.04 (3, 0.98) |
Case 3 | |||
Case 5 | |||
IgG (j) + Fib (i) | Case 1 | 1.06 ± 0.02 (3, 0.99) | 0.90 ± 0.01 (3, 1.00) |
Case 3 | |||
Case 5 | |||
FII (j) + Fib (i) | Case 1 | 1.19 ± 0.06 (3, 0.98) | 0.96 ± 0.06 (3, 0.96) |
Case 3 | |||
Case 5 | |||
FII (j) + IgG (i) | Case 1 | 1.07 ± 0.05 (3, 0.98) | 0.97 ± 0.06 (3, 0.96) |
Case 3 | |||
Case 5 |
Table 3B: Selectivity Factors for Reversed i,j Adsorption Competition to Octadecyltrichlorosilane-Treated Glass Particles (OTS) | |||
---|---|---|---|
Reversed Protein Pairs(from Table 2_OTS) | Experimental Condition | S̄i,j(N,R2) | Si,j(N,R2) |
HSA (j) + Fib (i) | Case 2 | 1.03 ± 0.08 (4, 0.91) | 1.09 ± 0.06 (4, 0.95) |
Case 4 | |||
Case 5 | |||
HSA (j) + IgG (i) | Case 2 | 1.09 ± 0.05 (4, 0.79) | 0.91 ± 0.04 (4, 0.86) |
Case 3 | |||
Case 5 |
Notes: See text for symbology and Section 3.2 for discussion of experimental conditions Cases 1–5. Column 4 is repeated from Table 2 for convenience of comparison.