Table 4.
Table 4A.: Characteristic Parameters obtained from Binary Protein Adsorption Kinetics to Octyl Sepharose (OS) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
State 1 | State 2 | ||||||||||
Protein Pairs | Experimental Condition | Di (mg/mL) | Dj (mg/mL) | ||||||||
HSA (i) + Fib (j) | Case 1 | 0.65 ± 0.04 | 0.66 ± 0.02 | 1.02 ± 0.10 | 0.70 ± 0.08 | 0.82 ± 0.09 | 0.42 ± 0.05 | ||||
Case 2 | 1.16 ± 0.07 | 1.64 ± 0.06 | 0.84 ± 0.07 | 0.74 ± 0.06 | 0.52 ± 0.03 | 0.45 ± 0.02 | |||||
Case 3 | 2.20 ± 0.13 | 1.08 ± 0.08 | 0.73 ± 0.06 | 0.56 ± 0.05 | 0.50 ± 0.05 | 0.42 ± 0.05 | |||||
Case 4 | 1.16 ± 0.07 | 3.36 ± 0.17 | 0.83 ± 0.07 | 0.77 ± 0.04 | 0.53 ± 0.05 | 0.60 ± 0.02 | |||||
Case 5 | 2.20 ± 0.13 | 3.36 ± 0.17 | 1.06 ± 0.07 | 0.85 ± 0.05 | 0.71 ± 0.05 | 0.64 ± 0.02 | |||||
HSA (i) + IgG (j) | Case 1 | 0.65 ± 0.04 | 0.55 ± 0.07 | 0.86 ± 0.07 | 0.94 ± 0.13 | 0.55 ± 0.04 | 0.64 ± 0.09 | ||||
Case 2 | 1.21 ± 0.07 | 2.31 ± 0.29 | 0.92 ± 0.07 | 0.95 ± 0.13 | 0.74 ± 0.06 | 0.68 ± 0.10 | |||||
Case 3 | 2.20 ± 0.13 | 2.31 ± 0.29 | 1.00 ± 0.07 | 0.87 ± 0.13 | 0.29 ± 0.05 | 0.32 ± 0.04 | |||||
Case 4 | 1.21 ± 0.07 | 4.44 ± 0.57 | 0.76 ± 0.05 | 0.91 ± 0.12 | 0.57 ± 0.03 | 0.62 ± 0.08 | |||||
Case 5 | 2.20 ± 0.13 | 4.44 ± 0.57 | 1.03 ± 0.12 | 0.98 ± 0.13 | 0.42 ± 0.07 | 0.47 ± 0.08 | |||||
HSA (i) + Ub (j) | Case 1 | 0.70 ± 0.04 | 0.33 ± 0.03 | 0.96 ± 0.06 | 2.00 ± 0.31 | 0.78 ± 0.03 | 1.45 ± 0.14 | ||||
Case 3 | 2.20 ± 0.13 | 1.22 ± 0.10 | 1.04 ± 0.07 | 0.82 ± 0.08 | 0.59 ± 0.04 | 0.49 ± 0.05 | |||||
Case 5 | 2.20 ± 0.13 | 2.19 ± 0.18 | 0.99 ± 0.14 | 1.14 ± 0.11 | 0.44 ± 0.05 | 0.39 ± 0.06 | |||||
IgG (i) + Fib (j) | Case 1 | 1.37 ± 0.17 | 0.80 ± 0.03 | 0.89 ± 0.12 | 0.69 ± 0.04 | 0.69 ± 0.09 | 0.51 ± 0.03 | ||||
Case 3 | 4.44 ± 0.57 | 1.36 ± 0.05 | 0.90 ± 0.18 | 0.71 ± 0.05 | 0.58 ± 0.11 | 0.52 ± 0.02 | |||||
Case 5 | 4.44 ± 0.57 | 3.36 ± 0.17 | 1.10 ± 0.20 | 0.91 ± 0.06 | 0.75 ± 0.14 | 0.68 ± 0.04 | |||||
FII (i) +Fib (j) | Case 1 | 0.12 ± 0.01 | 0.66 ± 0.02 | 6.17 ± 0.61 | 0.83 ± 0.05 | 2.58 ± 0.33 | 0.38 ± 0.06 | ||||
Case 3 | 2.01 ± 0.12 | 1.36 ± 0.05 | 1.52 ± 0.13 | 0.76 ± 0.08 | No State 2 | ||||||
Case 5 | 2.01 ± 0.12 | 3.36 ± 0.17 | 1.17 ± 0.08 | 0.83 ± 0.06 | 0.70 ± 0.10 | 0.55 ± 0.03 | |||||
FII (i) + IgG (j) | Case 1 | 0.12 ± 0.01 | 0.66 ± 0.08 | 5.33 ± 0.56 | 1.06 ± 0.14 | No State 2 | |||||
Case 3 | 2.01 ± 0.12 | 1.37 ± 0.17 | 1.29 ± 0.09 | 0.85 ± 0.12 | No State 2 | ||||||
Case 5 | 2.01 ± 0.12 | 4.44 ± 0.57 | 1.22 ± 0.10 | 0.86 ± 0.12 | 0.55 ± 0.05 | 0.45 ± 0.07 |
Table 4B: Characteristic Parameters obtained from Binary Protein Adsorption Kinetics to Octadecyltrichlorosilane-Treated Glass Particles (OTS) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
State 1 | State 2 | ||||||||||
Protein Pairs | Experimental Condition | Di (mg/mL) | Dj (mg/mL) | ||||||||
HSA (i) + Fib (j) | Case 2 | 0.53 ± 0.04 | 1.62 ± 0.19 | 0.19 ± 0.02 | 0.08 ± 0.01 | ||||||
Case 4 | 0.53 ± 0.04 | 2.50 ± 0.30 | 0.66 ± 0.09 | 0.32 ± 0.05 | 0.15 ± 0.04 | 0.05 ± 0.03 | |||||
Case 5 | 1.50 ± 0.12 | 2.50 ± 0.30 | 0.75 ± 0.08 | 0.71 ± 0.11 | 0.12 ± 0.03 | 0.22 ± 0.03 | |||||
HSA (i) + IgG (j) | Case 2 | 0.53 ± 0.04 | 0.67 ± 0.08 | 0.96 ± 0.09 | 1.28 ± 0.18 | 0.53 ± 0.10 | 0.66 ± 0.12 | ||||
Case 3 | 1.50 ± 0.12 | 1.95 ± 0.24 | 1.20 ± 0.11 | 1.44 ± 0.21 | 0.61 ± 0.07 | 0.88 ± 0.11 | |||||
Case 5 | 1.50 ± 0.12 | 2.71 ± 0.34 | 1.17 ± 0.14 | 1.48 ± 0.26 | 0.15 ± 0.04 | 0.29 ± 0.05 |
Notes: See text for symbology and Section 3.2 for discussion of experimental conditions Cases 1–5.