Figure 1.

Protein alignment of the aegerolysin family members. Sequences were retrieved from Entrez (http://www.ncbi.nlm.nih.gov/sites/gquery) and manually edited to include only the aegerolysin domain for an optimal alignment generated by M-Coffee.^25,26 The alignment output was edited using GeneDoc²⁷ conserved mode of shading, showing percentage of conservation (60%, light gray; 80%, grey; 100%, dark grey) within a column in the alignment. Consensus (boxed in red) generated by Jalview²⁸ was used to predicit aegerolysin secondary structure. The distribution of secondary structure elements is presented bellow the protein alignment (green box-extended strand, grey line - random coil). The accession numbers of the proteins in Figure 1 are as follows: aegerolysin (gi 24636240), ostreolysin (gi 60461919), pleurotolysin_A (gi 54312022), T_versicolor (gi 90639437), H_annosum (gi 186897694), Asp-hemolysin (gi 70985747), A_clavatus (gi 121709507), A_nidulans (gi 67522192), A_niger_1 (gi 145230219), A_niger_2 (gi 145256342), A_oryzae_1 (gi 169777319), A_oryzae_2 (gi 169772307), A_oryzae_3 (gi 169785219), A_terreus (gi 115390458), N_fischeri (gi 119487614), B_cinerea (gi 116087898), C_immitis (gi 119182290), P_brasiliensis (gi 46346063), N_crassa (gi 85079504), B_vulgaris (gi 26112720), L_multiflorum (gi 46507636), R_australe (gi 158524422), P_aeruginosa_1 (gi 152985646), P_aeruginosa_2 (gi 15595320), C_bifermentans_1 (gi 2292821), C_bifermentans_2 (gi 2292820), B_thuringiensis_1 (gi 14571542), B_thuringiensis_2 (gi 49175503).