Figure 8.

Antibodies can be eliminated from Western Blots by using T-Mod-GFP fusions. (A) Schematic of the detection reagent. Monomeric enhanced GFP (green) is fused to the C-terminus of T-Mod(MMY) (blue). (B) Duplicate gels were stained for total protein (left) or probed with T-Mod(MMY)-mEGFP (right). The lane on the right could be readily visualized using a 365 nm UV transilluminator typically used for DNA gels, and the image was captured with a digital camera. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]