Figure 3.

Comparison of the temperature dependence of tryptophan fluorescence spectroscopic signals for the holo- (holo-apo, in the absence –––, and holo in the presence ––– of 40 μM FAD) and apoprotein (- - - -) of hDAAO. All experiments were performed in 20 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 5% glycerol and 5 mM 2-mercaptoethanol, and at 0.1 mg/mL protein concentration. Spectral signals were monitored continuously during progressive heating from 20 to 80°C at a heating rate of 0.5°C/min and are given as percent of the total observed change regardless of the direction of the change.