Table I.

Amide Chemical Exchange Rates of CypA. Chemical Exchange Rates, Kex, were Calculated on a per Residue Basis Using Both 600 MHz and 900 MHz R2-CPMG Dispersion. Exchange Rates are not Reported for Those Residues Exhibiting Uncertainties Greater Than the Calculated Rates or for Those Residues Exhibiting Exchange <0.5 s⁻¹ at the Given Temperature

Residue	0°C	10°C	20°C
48	1456 ± 370
55	1159 ± 150	1176 ± 200	1584 ± 340
56	1070 ± 160		1239 ± 400
57	1511 ± 360
61			1354 ± 350
62	1534 ± 180	1542 ± 300
63	1456 ± 340	2417 ± 460	1957 ± 310
65	811 ± 200	2129 ± 400	5114 ± 1000
66	559 ± 180	2849 ± 230	5452 ± 1000
67	189 ± 260	3628 ± 450	4193 ± 1000
68	934 ± 350
69	725 ± 400
72		1200 ± 300	5794 ± 2800
73
74		3666 ± 350
75	748 ± 170
76	956 ± 100	2578 ± 120	5326 ± 1000
77	562 ± 150	2558 ± 160	5490 ± 1000
82	1070 ± 120	O.L.	2240 ± 250
83	633 ± 100	2610 ± 280	5201 ± 1000
84	1200 ± 400
91	kA = 0.54 ± 0.07a	kA = 0.85 ± 0.18a	960 ± 200
92	kA = 1.64 ± 0.15a	kA = 2.15 ± 0.11a	1072 ± 200
96	kA = 1.01 ± 0.11a	kA = 0.9 ± 0.15a	1351 ± 250
98	1159 ± 200	2130 ± 400	1031 ± 300
99	460 ± 100	400 ± 100	1320 ± 100
102	1511 ± 150	1524 ± 400	1750 ± 100
109	1269 ± 150	3041 ± 1000	1700 ± 600
110	684 ± 120	3142 ± 500	5800 ± 1000
111	1008 ± 100	2737 ± 200	4864 ± 1500
113	950 ± 100	1400 ± 200	1400 ± 100
119	kA = 1.84 ± 0.14a	kA = 1.41 ± 0.08a	1588 ± 200
120	kA = 1.85 ± 0.12a	kA = 2.74 ± 0.13a	1366 ± 100
122	kA = 1.84 ± 0.11a	kA = 2.33 ± 0.13a	1408 ± 200
126			1453 ± 160
128			1038 ± 240

^aOnly the forward rate constant, k_A, could be extracted due to slow exchange (see text). Both k_A and the respective uncertainties were calculated using the “slow exchange equation” described in Materials and Methods.